CJC-1295 (no DAC)
Short-acting GHRH analog often paired with ipamorelin for synergistic GH release.
🔬 Mechanism of Action
CJC-1295 without DAC, also known as Modified GRF(1-29) or Mod GRF, is a truncated and modified analog of GHRH (amino acids 1-29). The four amino acid substitutions protect it from enzymatic degradation, extending the biological half-life from ~7 minutes (native GHRH) to approximately 30 minutes.
Unlike the DAC version, Mod GRF creates sharp, discrete GH pulses that closely mimic natural physiology. This is why it is commonly paired with a ghrelin mimetic (like Ipamorelin) — the GHRH analog amplifies the pulse amplitude while the GHRP initiates the pulse, creating a synergistic effect that exceeds either peptide alone.
Source: PMID: 16352683
📜Background & History
CJC-1295 without DAC, commercially known as Modified GRF(1-29) or Mod GRF, is derived from the natural 44-amino-acid GHRH by retaining only the bioactive first 29 amino acids with four protective substitutions (Ala2→D-Ala, Gln8→Ala, Ala15→Ala, Leu27→Ala). These substitutions protect against enzymatic degradation by dipeptidyl peptidase-4 while preserving full receptor binding affinity. First described in research by Sato et al., it remains the preferred GHRH analog for physiological pulsatile GH protocols.
🎯 Research Use Cases
- ✓Pulsatile GH release protocols mimicking natural physiology
- ✓Pre-sleep GH pulse maximization for recovery and anabolism
- ✓Stacked with GHRPs for synergistic GH release
- ✓Body recomposition in GH-deficient adults
💉 Dosing Protocol
| Typical Dose | 100-300 mcg/injection |
| Frequency | 1-3× daily |
| Half-Life | ~30 minutes |
| Common Vial Sizes | 2 mg, 5 mg |
🧪 Reconstitution Example
⚠️Safety & Considerations
Research peptide. May cause facial flushing immediately after injection (common and transient). Should not be used by individuals with active cancer. Best used on an empty stomach for maximum GH pulse amplitude.
⚡Interactions & Contraindications
Must be injected on an empty stomach — insulin blunts the GH pulse. Avoid combining with CJC-1295 DAC (redundant mechanisms). Glucocorticoids suppress GH response. Evening dosing maximizes natural GH rhythm synchronization.
🔗Synergies & Common Stacks
The gold-standard GHRH+GHRP stack. Mod GRF amplifies pulse amplitude; Ipamorelin initiates the pulse cleanly without cortisol or prolactin elevation. Typically dosed 100 mcg + 100 mcg per injection.
More potent GH release than with Ipamorelin, at the cost of some cortisol and prolactin elevation. Good for anabolic-focused protocols.
Strongest hunger drive — used when caloric intake increase is desirable alongside GH elevation.
