CalcMyPeptide
Fat LossAlso known as: Anti-Obesity Drug 9604

AOD-9604

AOD-9604 (Anti-Obesity Drug 9604) is a highly precise, structurally modified fragment of human Growth Hormone (hGH), specifically isolated to replicate its fat-burning capability while stripping away all other endocrine effects. Formulated originally as an obesity intervention, it triggers immense lipolysis (fat destruction) and actively inhibits lipogenesis (new fat storage). Crucially, because it is an isolated fragment, it delivers these catastrophic fat-loss effects without altering IGF-1 levels, triggering cellular growth, or risking the severe insulin resistance classically associated with long-term hGH use.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Low2 peer-reviewed citations

Quick Stats

Half-Life~30 minutes (SC)
Dose Range300-500 mcg/day
Frequency1× daily (fasted)
Vial Sizes5 mg
BioavailabilitySubcutaneous injection or oral
Year Developed1990s

Scientific Data

Molecular Formula
C78H123N23O23S2
Molecular Weight
1816.12 g/mol
CAS Number
PubChem ID
Developer
Metabolic Pharmaceuticals (Melbourne, Australia)

Mechanism of Action

AOD-9604 is a modified fragment of human growth hormone (hGH amino acids 177-191, with an added tyrosine) specifically engineered to retain hGH's fat-metabolizing properties while eliminating its anabolic and proliferative effects.

AOD-9604 stimulates lipolysis (fat breakdown) and inhibits lipogenesis (fat storage) via β-3 adrenergic receptors and direct lipase activation, without binding the GH receptor or elevating IGF-1. This selective fat-burning mechanism makes it unique among peptides — it lacks the cancer risk and metabolic side effects associated with GH receptor agonism. It was developed by Metabolic Pharmaceuticals (Melbourne) and reached Phase IIb/III clinical trials for obesity.

Source: PMID: 11713833

Background & History

AOD-9604 is a modified fragment of human growth hormone consisting of amino acids 176–191, with the addition of a tyrosine residue at the N-terminus (making it AOD rather than just fragment 176-191). Developed by Monash University researchers led by John Ng in Australia, it was designed to capture the lipolytic properties of GH without its diabetogenic effects. Phase III trials for obesity (Metabolic) were conducted in 2003–2007 but did not meet primary endpoints in the broadest population despite showing fat-specific effects. It received GRAS (Generally Recognized as Safe) status in the US.

Research Use Cases

  • Targeted fat loss without effects on blood glucose or IGF-1
  • Body recomposition with minimal metabolic side effects
  • Adjunct to GLP-1 protocols for fat oxidation support

Dosing Protocol

Typical Dose300-500 mcg/day
Frequency1× daily (fasted)
Half-Life~30 minutes (estimated)
Common Vial Sizes5 mg

Dosing Protocols

Fat Loss Protocol

Dose
200 - 400 mcg
Frequency
Daily SC injection (fasted AM)
Note: Fasted injection maximizes lipolytic effect. Often cycled 5 days on / 2 days off or continuous for 12 weeks.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 300500 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)231 mcg309 mcg386 mcg
140 lb(63 kg)270 mcg360 mcg450 mcg
160 lb(73 kg)313 mcg417 mcg521 mcg
180 lb(82 kg)351 mcg469 mcg586 mcg
200 lb(91 kg)390 mcg520 mcg650 mcg
220 lb(100 kg)429 mcg571 mcg714 mcg
250 lb(113 kg)484 mcg646 mcg807 mcg

💉 For exact syringe units based on your vial concentration, use the AOD-9604 Reconstitution Calculator →

Administration

Route
Subcutaneous injection
Timing
Fasted, 30-60 min before AM exercise or first meal.
Fasting Required?
Yes — inject on an empty stomach

Expected Timeline

Week 1-4
Enhanced fat oxidation during fasted state. Increased energy expenditure.
Month 2-3
Measurable body fat reduction, particularly in stubborn subcutaneous depots.

Who Is It For?

Fat Loss / Lipolysis

Low

Selectively targets fat metabolism. No IGF-1 elevation or GH receptor agonism. Phase IIb trials showed modest fat loss.

Reconstitution Example

Vial
5 mg
Water
2 mL
Concentration
2.5 mg/mL
Per Unit (100u syringe)
25 mcg
Dose of 300 mcg = 12 units on a 100-unit insulin syringe

Safety & Considerations

GRAS status (Generally Recognized As Safe) for oral use in some jurisdictions. Well-tolerated in clinical trials. Does not elevate IGF-1 or blood glucose. No known anabolic or proliferative effects.

Regulatory & Legal Status

FDA Status (US)
Research Only

Reached Phase 3 trials for obesity; not FDA-approved as a drug

WADA Status (2026)
Not Listed

Not currently on the WADA 2026 Prohibited List. Policies may change — verify before competition.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

Does not affect IGF-1 or blood glucose — safer metabolically than full GH. No significant drug interactions identified. Not for use with active cancer.

Synergies & Common Stacks

GLP-1 reduces appetite and caloric intake; AOD-9604 independently stimulates lipolysis. Complementary fat loss mechanisms operating through different pathways.

Tesamorelin reduces visceral fat via GH; AOD-9604 targets subcutaneous and visceral fat directly. Additive lipolytic effects.

Dosing Quick Reference

AOD-9604— Dosing Guide
Dose Range
300-500 mcg/day
Half-Life
~30 minutes (estimated)
Frequency
1× daily (fasted)
Route
Subcutaneous
5 mg vial
💧 2 mL BAC water📐 2.5 mg/mL concentration💉 25 mcg/unit (100u syringe)
Fat Losscalcmypeptide.com

Frequently Asked Questions

Does AOD-9604 raise IGF-1?
No — this is its key advantage. AOD-9604 is a fragment of GH that retains lipolytic effects without binding the GH receptor. It does not raise IGF-1, blood glucose, or cause the proliferative effects associated with GH use.
Should AOD-9604 be injected fasted?
Yes — fasted administration maximizes lipolytic effect. Inject 30-60 minutes before exercise or breakfast. Insulin blunts lipolysis, so avoid carbohydrate intake around injection time.

References

  1. Heffernan M et al. "A fragment of GH retains lipolytic activity without mitogenic effects".” Journal of Endocrinology (2001). PMID: 11520032

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