Healing & Recovery

Follistatin 315

FST-315 · FST315 · circulating follistatin

The circulating splice of follistatin (315 aa) versus FST-344, which we already list. FST-315 carries the acidic C-terminal tail that keeps it in plasma; FST-344 is the tissue-associated form. Gray-market vials labeled 315 vs 344 are isoform SKUs of the same myostatin/activin-binding protein — not two drugs. Neither is ACE-031 (a discontinued ActRIIB fusion).

Not FDA-approved — research chemicalWADA (S4) — prohibited in sport at all times
Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Low1 peer-reviewed citation
Typical research dose
100-300 mcg/day (research; same ballpark as FST-344 community use)
Frequency
1× daily for short research cycles
Half-life
Hours-class circulating protein (SKU PK unpublished)
Common vials
1 mg
CAS
N/A (isoform SKU)
Molecular weight
~31–35 kDa class (glycoprotein)
Dose it
1 mg + 1 mL BAC water1 mg/mL · 100 mcg ≈ 10 units (U-100)
Open in calculator

Mechanism of Action

Follistatin-315 is the circulating splice (acidic C-terminal tail). Follistatin-344 is the tissue-associated form we already list. Both bind myostatin and activins at ActRIIB. Gray-market “315” vs “344” vials are isoform SKUs — identity is often unverified.

ACE-031 / ramatercept was a different molecule (ActRIIB fusion), discontinued. Do not treat FST-315 as that program.

Source: Follistatin biology PMID: 17108139; isoform distinction is splice-form biochemistry, not a 315-specific human trial

Isoform, not a new drug

315 vs 344 changes where the protein hangs out more than what it binds. WADA treats myostatin inhibitors as S4. Research powder is not a clinical myostatin drug.

Dosing Protocol

Typical Dose100-300 mcg/day (research; same ballpark as FST-344 community use)
Frequency1× daily for short research cycles
Half-Life~hours (circulating isoform; human peptide-SKU PK unpublished)
Common Vial Sizes1 mg

Dosing Protocols

Research (community, unlabeled)

Dose
100–300 mcg/day
Frequency
Short cycles
Note: Copied from FST-344 forums. Not a label. Myostatin-inhibitor risk profile.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 100300 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)77 mcg154 mcg231 mcg
140 lb(63 kg)90 mcg180 mcg270 mcg
160 lb(73 kg)104 mcg209 mcg313 mcg
180 lb(82 kg)117 mcg234 mcg351 mcg
200 lb(91 kg)130 mcg260 mcg390 mcg
220 lb(100 kg)143 mcg286 mcg429 mcg
250 lb(113 kg)161 mcg323 mcg484 mcg

💉 For exact syringe units based on your vial concentration, use the Follistatin 315 Reconstitution Calculator →

Administration

Route
Subcutaneous (research)
Timing
Not established.
Fasting Required?
No — food timing not critical

Expected Timeline

Unknown in humans
No honest consumer timeline. Muscle-wasting drug programs are not this vial.

Who Is It For?

Isoform identity

Low

Listed so 315 search does not 404. Prefer the 344 leaf for the class story; this page is the circulating splice.

Reconstitution Example

Vial
1 mg
Diluent
1 mL
Concentration
1 mg/mL
Per Unit (100u syringe)
10 mcg
Dose of 100 mcg = 10 units on a 100-unit insulin syringe

Safety & Considerations

Myostatin/activin blockade is not a casual recovery peptide. Theoretical fertility, vascular, and off-target TGF-β effects. WADA S4. Research-only.

Regulatory & Legal Status

FDA Status (US)
Research Only

Circulating follistatin isoform. Not ACE-031. No FDA myostatin-inhibitor approval

WADA Status (2026)
Prohibited (S4)

Competitive athletes subject to anti-doping controls should not use Follistatin 315.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Follistatin 315 vs. Follistatin 344

AttributeFollistatin 315Follistatin 344
IsoformCirculating (acidic C-tail)Tissue-associated splice
LigandsMyostatin / activins (same class)Myostatin / activins (same class)
SKU realityOften unverified gray-market powderSame problem

Verdict: 315 vs 344 is splice localization, not two mechanisms. Neither is ACE-031.

Dosing Quick Reference

Follistatin 315— Dosing Guide
Dose Range
100-300 mcg/day (research; same ballpark as FST-344 community use)
Half-Life
~hours (circulating isoform; human peptide-SKU PK unpublished)
Frequency
1× daily for short research cycles
Route
Subcutaneous
1 mg vial
💧 1 mL BAC water📐 1 mg/mL💉 10 mcg/unit (100u syringe)
Healing & Recoverycalcmypeptide.com

Frequently Asked Questions

Is 315 stronger than 344?
It is the circulating isoform. “Stronger” is vendor copy. Ligand class is the same.
Is this ACE-031?
No. ACE-031 was an ActRIIB-Fc fusion, discontinued. Different molecule.

References

  1. Lee SJ et al. lineage Follistatin / myostatin biology (class).” PNAS / related (2007). PMID: 17108139

Looking for a trusted source? See our recommended suppliers →

Independently tested · COA-verified · Save 10% with our exclusive code