CalcMyPeptide
Skin & CosmeticAlso known as: Copper peptide, Glycyl-L-histidyl-L-lysine:copper(II)

GHK-Cu

GHK-Cu (Glycyl-L-histidyl-L-lysine) is a legendary, naturally occurring copper-binding tripeptide universally recognized as the gold standard in dermal remodeling and anti-aging cosmetics. Functioning as a profound epigenetic modulator, GHK-Cu quite literally reverses the biological age of human skin by upregulating the expression of over 4,000 distinct genes. Administered subcutaneously or topically, it aggressively eradicates wrinkles, heals catastrophic scars, restores hair follicle vascularity, and acts as a massive systemic anti-inflammatory agent.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Moderate3 peer-reviewed citations

Quick Stats

Half-Life~<30 minutes (systemic)
Dose Range1-3 mg/day (SC) or topical
Frequency1× daily
Vial Sizes5 mg, 50 mg
BioavailabilityTopical and subcutaneous
Year Developed1973

Scientific Data

Molecular Formula
C14H24CuN6O4
Molecular Weight
340.83 g/mol (copper complex)
CAS Number
PubChem ID
Developer
Loren Pickart (originally isolated)

Mechanism of Action

GHK-Cu (Glycyl-L-histidyl-L-lysine:copper(II)) is a naturally occurring copper tripeptide found in human plasma, saliva, and urine. Plasma levels decline significantly with age — from ~200 ng/mL at age 20 to ~80 ng/mL by age 60. GHK-Cu has broad biological activities: it stimulates collagen synthesis (Types I and III), glycosaminoglycan production (chondroitin sulfate, dermatan sulfate), and decorin synthesis.

GHK-Cu is unique in being effective both topically and systemically. It promotes wound healing, skin remodeling, and hair growth by enlarging hair follicles and extending the anagen (growth) phase. Studies show similar efficacy to minoxidil 5% for stimulating hair growth, with additional skin-quality benefits.

Source: PMID: 18492131

Background & History

GHK-Cu (Glycyl-L-Histidyl-L-Lysine:copper) was discovered by Loren Pickart in 1973 while investigating why liver cells of aged humans behaved differently from young plasma. He isolated GHK as the causative factor in youthful plasma that reprogrammed aged cells. The copper complex GHK-Cu is found naturally in human plasma, saliva, and urine, declining with age. It is one of the most pleiotropic peptides known — activating over 4,000 genes — and has been called a "master skin repair signal." Topical cosmetic use is widespread; injectable research use is growing.

Research Use Cases

  • Skin rejuvenation: collagen synthesis, wrinkle reduction, wound healing
  • Hair growth stimulation and follicle regeneration
  • Anti-inflammatory via TNF-α, IL-6 suppression
  • Tissue repair: lung, bone, liver remodeling in animal models
  • Neuroprotection via BDNF upregulation

Dosing Protocol

Typical Dose1-3 mg/day (SC) or topical
Frequency1× daily
Half-Life~1-2 hours (estimated)
Common Vial Sizes5 mg, 50 mg

Dosing Protocols

Injectable Protocol

Dose
1 - 3 mg
Frequency
Daily (SC)
Note: For systemic tissue repair, wound healing, and anti-aging. Rotate injection sites.

Topical Application

Dose
0.01 - 0.1% concentration
Frequency
Once or twice daily (topical)
Note: Widely used in cosmetic serums. Do not combine with vitamin C or AHAs in topical use.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 10003000 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)771 mcg1543 mcg2314 mcg
140 lb(63 kg)900 mcg1800 mcg2700 mcg
160 lb(73 kg)1043 mcg2086 mcg3129 mcg
180 lb(82 kg)1171 mcg2343 mcg3514 mcg
200 lb(91 kg)1300 mcg2600 mcg3900 mcg
220 lb(100 kg)1429 mcg2857 mcg4286 mcg
250 lb(113 kg)1614 mcg3229 mcg4843 mcg

💉 For exact syringe units based on your vial concentration, use the GHK-Cu Reconstitution Calculator →

Administration

Route
Subcutaneous injection or topical application
Timing
Anytime. No fasting required.
Fasting Required?
No — food timing not critical

Expected Timeline

Week 2-4
Improved skin texture, hair follicle stimulation, wound healing acceleration.
Month 1-3
Collagen I and III synthesis increases. Hair growth in anagen phase extended.

Who Is It For?

Skin Anti-Aging / Collagen

High

One of the most studied cosmetic peptides. Direct collagen I/III synthesis and wound healing.

Hair Growth

Moderate

Enlarges follicles and extends anagen phase. Studies show efficacy comparable to minoxidil 5%.

Reconstitution Example

Vial
50 mg
Water
5 mL
Concentration
10 mg/mL
Per Unit (100u syringe)
100 mcg
Dose of 1000 mcg = 10 units on a 100-unit insulin syringe

Safety & Considerations

Naturally occurring human peptide with excellent safety profile. Both injectable and topical formulations are well-established. Do not combine topically with vitamin C or AHAs.

Regulatory & Legal Status

FDA Status (US)
Research Only
WADA Status (2026)
Not Listed

Not currently on the WADA 2026 Prohibited List. Policies may change — verify before competition.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

Topical formulations: minimal systemic absorption — extremely safe. Injectable: copper accumulation possible with excessive doses (keep to research protocols). Do not use concurrently with copper chelators (e.g., penicillamine). Very favorable safety profile at therapeutic doses.

Synergies & Common Stacks

BPC-157 drives angiogenesis and growth factor expression; GHK-Cu amplifies collagen synthesis and dermal matrix remodeling. Complementary wound healing and skin repair synergy.

TB-500 (actin regulation) + GHK-Cu (collagen/matrix synthesis) together support comprehensive connective tissue restoration.

GHK-Cu vs. BPC-157

AttributeGHK-CuBPC-157
MechanismCopper-peptide signaling + collagen synthesisVEGF/FGF angiogenesis + NO pathway
Admin RouteTopical (primary), injectableInjectable (primary), oral (gut)
Best ForSkin aging, wound healing, hair, topical collagenTendon, ligament, gut, systemic injury healing
Evidence QualityLimited human data (mostly in-vitro)Animal models (robust preclinical)
AvailabilityOTC creams + injectable research gradeInjectable or oral research grade

Verdict: GHK-Cu excels in topical applications for skin, hair, and superficial wound healing. BPC-157 is the preferred option for deep tissue, joint, and GI injury repair. Many longevity protocols combine both.

Dosing Quick Reference

GHK-Cu— Dosing Guide
Dose Range
1-3 mg/day (SC) or topical
Half-Life
~1-2 hours (estimated)
Frequency
1× daily
Route
Subcutaneous
5 mg vial50 mg vial
💧 5 mL BAC water📐 10 mg/mL concentration💉 100 mcg/unit (100u syringe)
Skin & Cosmeticcalcmypeptide.com

Frequently Asked Questions

Can GHK-Cu be used topically or only by injection?
Both — effective topically for skin repair, anti-aging, and hair growth, and systemically (SC injection) for broader tissue repair. Topical: 0.01-0.1%. Injectable: 1-3 mg/day SC.
Does GHK-Cu help with hair growth?
Yes — GHK-Cu enlarges hair follicles and extends the anagen phase. Studies show similar efficacy to minoxidil 5% with additional skin benefits.

References

  1. Pickart L et al. "GHK-Cu and skin rejuvenation".” Journal of Aging Science (2015). PMID: 26844265

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