Skin & Cosmetic

GHK-Cu

Copper peptide · Glycyl-L-histidyl-L-lysine:copper(II)

Gly-His-Lys copper complex. Pickart dermal/matrikine literature and topical cosmetics are the honest file. “4000 genes / reverse biological age” is marketing. Injectable research vials are a different copper exposure than a cream.

Not FDA-approved - research chemical
Reviewed by CalcMyPeptide Editorial Team
Last updated: August 2026Evidence: Moderate3 peer-reviewed citations
Typical research dose
1-3 mg/day (SC) or topical
Frequency
1× daily
Half-life
~<30 minutes (systemic)
Common vials
5 mg / 50 mg
CAS
89030-95-5
Molecular weight
340.83 g/mol (copper complex)

Use-case scores

0–10 against the evidence we have, not a gym ranking. Hidden domains do not apply here.

  • Skin / cosmetic5/10

    Pickart literature and topical cosmetic use are real. Gene-count marketing (“4000 genes”) is not a wrinkle RCT. Multi-lab dermal work, haircut for injectable hype: 5.

Compound card

Sequence
GHK·Cu2+
Class
Copper-binding matricryptic tripeptide (cosmetic + research injectable)
Formula
C14H24CuN6O4
Status
Not FDA-approved · research use
Dose it
50 mg + 5 mL BAC water → 10 mg/mL · 1000 mcg ≈ 10 units (U-100)
Open in calculator

How it works

GHK-Cu (Glycyl-L-histidyl-L-lysine:copper(II)) is a naturally occurring copper tripeptide found in human plasma, saliva, and urine. Plasma levels decline significantly with age - from ~200 ng/mL at age 20 to ~80 ng/mL by age 60. GHK-Cu has broad biological activities: it stimulates collagen synthesis (Types I and III), glycosaminoglycan production (chondroitin sulfate, dermatan sulfate), and decorin synthesis.

GHK-Cu is unique in being effective both topically and systemically. It promotes wound healing, skin remodeling, and hair growth by enlarging hair follicles and extending the anagen (growth) phase. Studies show similar efficacy to minoxidil 5% for stimulating hair growth, with additional skin-quality benefits.

Source: PMID: 18492131

Background & History

GHK-Cu (Glycyl-L-Histidyl-L-Lysine:copper) was discovered by Loren Pickart in 1973 while investigating why liver cells of aged humans behaved differently from young plasma. He isolated GHK as the causative factor in youthful plasma that reprogrammed aged cells. The copper complex GHK-Cu is found naturally in human plasma, saliva, and urine, declining with age. It is one of the most pleiotropic peptides known - activating over 4,000 genes - and has been called a "master skin repair signal." Topical cosmetic use is widespread; injectable research use is growing.

Research Use Cases

  • ✓Identity: copper tripeptide vs GHK Basic vs topical vs SubQ
  • ✓Why “4000 genes” is not a wrinkle RCT
  • ✓Not Botox and not a telomere drug

Dosing

PhaseDoseFrequencyNotes
Injectable Protocol1 - 3 mgDaily (SC)For systemic tissue repair, wound healing, and anti-aging. Rotate injection sites.
Topical Application0.01 - 0.1% concentrationOnce or twice daily (topical)Widely used in cosmetic serums. Do not combine with vitamin C or AHAs in topical use.

Administration

Route / form
Subcutaneous injection or topical application
Timing
Anytime. No fasting required.
Empty stomach?
No - Food timing is not critical.

GHK-Cu: Topical serum / cream vs Injectable research vial

Topical serum / cream
Bioavailability
Cosmetic and explant literature is the honest topical file (Pickart dermal/matrikine work, PMID 18492131). That is not a published systemic F% for a face serum, and it is not Botox.
Dose note
Typical cosmetic band is about 0.01–0.1% in a leave-on product, 1–2× daily. Do not combine in the same routine with vitamin C or AHAs (copper redox / irritation). No reconstitution calculator for a cream.
Advantages
  • This is how most people actually buy GHK-Cu.
  • No syringe. Blue color is the complex, not a potency assay by eye.
Considerations
  • “4000 genes / reverse biological age” is marketing.
  • Topical cosmetic trials are not an injectable wound-drug NDA.
Injectable research vial
Bioavailability
Systemic copper-peptide exposure from a reconstituted cake is a different job than a cream. Human injectable PK is thin. PIP and copper load are the practical limits, not a Pickart gene-chip headline.
Dose note
Community research band on this leaf: 1–3 mg SC daily from a reconstituted vial. BAC water. Blue solution is expected for the copper complex. Do not inject a topical serum.
Advantages
  • Vial math is checkable when the bottle is lyophilized GHK-Cu.
  • Separates systemic research use from cosmetic SKUs.
Considerations
  • Injectable lots are mostly preclinical plus PIP. Not a reconstruction NDA.
  • Copper exposure is real. This is not copper-free GHK Basic (separate leaf).
Recommendation: GHK-Cu: cream is the cosmetic SKU; SubQ is a different copper exposure from a research vial. Do not inject a serum or spread a cake on your face and call it the same protocol. Gene-count marketing is not a label.

Timeline

Week 2-4
Improved skin texture, hair follicle stimulation, wound healing acceleration.
Month 1-3
Collagen I and III synthesis increases. Hair growth in anagen phase extended.

Who it is for

Skin Anti-Aging / Collagen

High

One of the most studied cosmetic peptides. Direct collagen I/III synthesis and wound healing.

Hair Growth

Moderate

Enlarges follicles and extends anagen phase. Studies show efficacy comparable to minoxidil 5%.

Reconstitution

VialWaterConcentrationExample dose
50 mg lyophilized5 mL BAC10 mg/mL1 mg on 50 mg / 5 mL (10 mg/mL) = 0.10 mL = 10 units on a U-100. Blue solution is expected.

Change vial size or water volume? Open the reconstitution calculator.

Safety & Considerations

Copper tripeptide. Injectable lots are mostly preclinical plus PIP. Topical cosmetic trials exist. Do not combine topically with vitamin C or AHAs. Not a reconstruction NDA.

Regulatory & Legal Status

FDA Status (US)
Research Only
WADA Status (2026)
Not Listed

Not currently on the WADA 2026 Prohibited List. Policies may change - verify before competition.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Limits of current evidence

  • Most gene-expression work is in vitro / explant. Do not treat that as a human aging trial.
  • Copper load is a real constraint on high-mg daily SubQ folklore.
  • GHK Basic (copper-free) is a different SKU.

Verdict

GHK-Cu is Gly-His-Lys with copper. Topical cosmetic use is the honest mass-market story. Injectable research vials are a different exposure. It is not a telomere drug and not a Botox.

Interactions & Contraindications

Topical formulations: minimal systemic absorption - extremely safe. Injectable: copper accumulation possible with excessive doses (keep to research protocols). Do not use concurrently with copper chelators (e.g., penicillamine). Very favorable safety profile at therapeutic doses.

Synergies & Common Stacks

BPC-157 drives angiogenesis and growth factor expression; GHK-Cu amplifies collagen synthesis and dermal matrix remodeling. Complementary wound healing and skin repair synergy.

Actin-fragment marketing plus copper-tripeptide marketing. Complementary labels are not connective-tissue restoration and not a combo trial.

GHK-Cu vs. BPC-157

Canonical comparison: GHK-Cu vs BPC-157

AttributeGHK-CuBPC-157
MechanismCopper-peptide signaling + collagen synthesisVEGF/FGF angiogenesis + NO pathway
Admin RouteTopical (primary), injectableInjectable (primary), oral (gut)
Best ForSkin aging, wound healing, hair, topical collagenTendon, ligament, gut, systemic injury healing
Evidence QualityLimited human data (mostly in-vitro)Animal models (robust preclinical)
AvailabilityOTC creams + injectable research gradeInjectable or oral research grade

Verdict: GHK-Cu excels in topical applications for skin, hair, and superficial wound healing. BPC-157 is the preferred option for deep tissue, joint, and GI injury repair. Many longevity protocols combine both.

Frequently Asked Questions

Topical or injectable?▼
Both SKUs exist. Creams are the cosmetic job (about 0.01–0.1%). Injectable research vials are a different copper exposure (community 1–3 mg SC). Do not inject a serum. Gene-count marketing is not a label. Copper-free GHK is a separate leaf.
Can GHK-Cu be used topically or only by injection?▼
Both - effective topically for skin repair, anti-aging, and hair growth, and systemically (SC injection) for broader tissue repair. Topical: 0.01-0.1%. Injectable: 1-3 mg/day SC.
Does GHK-Cu help with hair growth?▼
Yes - GHK-Cu enlarges hair follicles and extends the anagen phase. Studies show similar efficacy to minoxidil 5% with additional skin benefits.

References

  1. Pickart L et al. “"GHK-Cu and skin rejuvenation".” Journal of Aging Science (2015). PMID: 26844265
  2. Pickart L, Margolina A. “GHK peptide as a natural modulator of multiple cellular pathways in skin biology.” Biomed Res Int (2015). PMID: 26236730
  3. Pickart L. “The human tripeptide GHK-Cu in prevention and treatment of skin aging.” Int J Cosmet Sci (2012). PMID: 22486774

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