CalcMyPeptide
Healing & RecoveryAlso known as: GnRH, LHRH, Luteinizing Hormone-Releasing Hormone

Gonadorelin

Gonadorelin (GnRH) is the bioidentical, synthetic equivalent of Gonadotropin-Releasing Hormone, functioning as the absolute cornerstone of the Hypothalamic-Pituitary-Gonadal (HPG) axis. In anti-aging protocols and Endocrinology, it has largely replaced hCG to preserve testicular volume, maintain high-quality spermatogenesis, and uphold endogenous testosterone production in men undergoing aggressive systemic TRT. Its half-life is excruciatingly short, meaning it demands highly calculated, pulsatile dosing schedules to aggressively stimulate, rather than accidentally suppress, the hormonal cascade.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: High2 peer-reviewed citations

Quick Stats

Half-Life~2-4 minutes (IV); ~10-40 minutes (SC)
Dose Range100-500 mcg/day
Frequency1-2× daily
Vial Sizes2 mg
BioavailabilitySubcutaneous injection (preferred for TRT co-administration)
Year Developed1971

Scientific Data

Molecular Formula
C55H75N17O13
Molecular Weight
1182.31 g/mol
CAS Number
PubChem ID

Mechanism of Action

Gonadorelin is a bioidentical synthetic version of the naturally occurring Gonadotropin-Releasing Hormone (GnRH/LHRH). It is a decapeptide that acts on anterior pituitary gonadotroph cells to stimulate pulsatile release of both LH (luteinizing hormone) and FSH (follicle-stimulating hormone).

In pulsatile administration, gonadorelin maintains and restores the hypothalamic-pituitary-gonadal (HPG) axis. It is prescribed alongside TRT (testosterone replacement therapy) to preserve fertility and testicular function. Continuous (non-pulsatile) administration paradoxically suppresses LH/FSH through GnRH receptor downregulation — this is exploited clinically by GnRH agonists like leuprolide for prostate cancer and endometriosis treatment.

Source: PMID: 1903034

Background & History

Gonadorelin is the synthetic form of endogenous GnRH (Gonadotropin-Releasing Hormone), a decapeptide produced in the hypothalamus that drives pulsatile release of LH and FSH from the pituitary. It was first synthesized and characterized by Andrew Schally and Roger Guillemin in 1971, earning them the 1977 Nobel Prize in Physiology or Medicine. Pulsatile gonadorelin maintains testicular function and testosterone production during androgen therapy by preserving the HPG axis — specifically preventing the testicular atrophy and infertility caused by exogenous testosterone.

Research Use Cases

  • HPG axis preservation during testosterone replacement therapy (TRT)
  • Male infertility treatment via LH/FSH stimulation
  • Post-cycle therapy (PCT) to restore natural testosterone production
  • Female fertility stimulation in hypothalamic amenorrhea

Dosing Protocol

Typical Dose100-500 mcg/day
Frequency1-2× daily
Half-Life~4 minutes
Common Vial Sizes2 mg

Dosing Protocols

TRT Co-Administration

Dose
100 mcg
Frequency
Twice weekly (SC) — same days as testosterone injection
Note: Maintain pulsatile HPG axis signaling during TRT to preserve testicular size and fertility.

PCT / Restart Protocol

Dose
200 - 500 mcg
Frequency
2-3x daily for 2-4 weeks (SC)
Note: Short pulsatile doses for HPTA restart post-cycle. Must be pulsatile — not continuous.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 100500 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)77 mcg231 mcg386 mcg
140 lb(63 kg)90 mcg270 mcg450 mcg
160 lb(73 kg)104 mcg313 mcg521 mcg
180 lb(82 kg)117 mcg351 mcg586 mcg
200 lb(91 kg)130 mcg390 mcg650 mcg
220 lb(100 kg)143 mcg429 mcg714 mcg
250 lb(113 kg)161 mcg484 mcg807 mcg

💉 For exact syringe units based on your vial concentration, use the Gonadorelin Reconstitution Calculator →

Administration

Route
Subcutaneous injection
Timing
Same time as TRT administration or morning for PCT.
Fasting Required?
No — food timing not critical

Expected Timeline

Day 1-7
LH/FSH secretion maintained or restored. Testicular blood flow preserved.
Week 2-8 (PCT)
HPG axis recovery. Rising testosterone from resumed LH stimulation of Leydig cells.

Who Is It For?

TRT Fertility Preservation

High

Gold standard for maintaining HPG axis during TRT. Prevents testicular atrophy and preserves fertility.

Post-Cycle Therapy (PCT)

High

Pulsatile dosing restarts LH/FSH production after suppression. FDA-approved (Factrel) with decades of data.

Reconstitution Example

Vial
2 mg
Water
2 mL
Concentration
1 mg/mL
Per Unit (100u syringe)
10 mcg
Dose of 100 mcg = 10 units on a 100-unit insulin syringe

Safety & Considerations

FDA-approved (Factrel) for diagnostic use with excellent safety profile. Must be administered in pulsatile fashion — continuous use will paradoxically suppress the HPG axis. Injection site irritation is the most common side effect.

Regulatory & Legal Status

FDA Status (US)
Approved

FDA-approved as Factrel®; widely used off-label as Gonadorelin Acetate in TRT protocols

WADA Status (2026)
Prohibited (S4)

Competitive athletes subject to anti-doping controls should not use Gonadorelin.

Classification

Compounded Drug (Rx)

US Compounding: Available via licensed pharmacy Rx

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

Pulsatile administration (every 60-90 min) is required for efficacy — continuous infusion paradoxically suppresses LH/FSH (basis of GnRH agonist therapy for prostate cancer). Depot formulations of GnRH agonists (leuprolide, histrelin) work by this paradoxical suppression mechanism. Antiandrogens blunt downstream testosterone response.

Synergies & Common Stacks

Kisspeptin acts upstream of GnRH — it's the master activator of GnRH neurons. Combining both ensures complete HPG axis stimulation from hypothalamic to pituitary to gonadal level.

Dosing Quick Reference

Gonadorelin— Dosing Guide
Dose Range
100-500 mcg/day
Half-Life
~4 minutes
Frequency
1-2× daily
Route
Subcutaneous
2 mg vial
💧 2 mL BAC water📐 1 mg/mL concentration💉 10 mcg/unit (100u syringe)
Healing & Recoverycalcmypeptide.com

Frequently Asked Questions

Why is gonadorelin used with TRT?
TRT suppresses LH/FSH production, causing testicular atrophy and infertility. Gonadorelin maintains pituitary LH/FSH output, preserving both testicular size and sperm production during TRT.
How often should gonadorelin be injected?
Typically 100-500 mcg subcutaneously, 2-3 times per week — or 2x per day for PCT. The key is pulsatile dosing — continuous exposure paradoxically suppresses the HPG axis.

References

  1. Schally AV et al. "Gonadorelin (GnRH) in clinical practice".” Endocrine Reviews (1980). PMID: 6774397

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