CalcMyPeptide
Weight ManagementAlso known as: LY3437943

Retatrutide

Retatrutide is an experimental, hyper-potent "triple-G" agonist representing the absolute cutting edge of metabolic peptide engineering. Currently dominating late-stage clinical trials, this multi-receptor peptide breaks all previous pharmaceutical weight-loss records, driving an astonishing 24.2% total body weight reduction over 48 weeks. By introducing a glucagon target alongside GLP-1 and GIP mechanisms, Retatrutide uniquely forces the body to rapidly burn stored liver fat (resolving NAFLD in nearly 90% of subjects) and dramatically upregulates baseline metabolic expenditure.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Emerging3 peer-reviewed citations

Quick Stats

Half-Life144 hours (~6 days)
Dose Range1-12 mg/week
Frequency1× weekly
Vial Sizes5 mg, 10 mg
Bioavailability~80% (subcutaneous)
Year Developed2020

Scientific Data

Molecular Formula
C221H342N46O68
Molecular Weight
4731.34 g/mol
CAS Number
PubChem ID

Mechanism of Action

Retatrutide (LY3437943) is a triple agonist peptide that simultaneously activates GLP-1, GIP, and glucagon receptors — making it the first triple-action incretin therapy. While GLP-1 and GIP agonism provide the established weight loss mechanisms (appetite suppression, insulin sensitization, gastric slowing), the addition of glucagon receptor activation introduces a third metabolic lever: increased hepatic fatty acid oxidation and energy expenditure.

Phase II trial data demonstrated up to 24.2% body weight reduction at 12 mg, already surpassing semaglutide and tirzepatide. In December 2025, Phase III TRIUMPH-4 results (68 weeks, adults with obesity + knee osteoarthritis) showed 28.7% weight loss at the 12 mg dose and 26.4% at 9 mg — setting a new benchmark for any pharmacological weight loss agent. Retatrutide has a 6-day half-life, supporting once-weekly dosing.

Source: PMID: 37351564 (Phase II)

Background & History

Retatrutide (LY3437943) represents the third generation of incretin therapy — a triple agonist targeting GLP-1, GIP, and glucagon receptors simultaneously. Developed by Eli Lilly, Phase II results (NEJM 2023) reported 24.2% weight reduction at 48 weeks with 12 mg dose — the highest ever recorded for a pharmacological agent. The glucagon component uniquely increases hepatic fat oxidation and energy expenditure, a mechanism absent in dual agonists. Phase III TRIUMPH trials are ongoing as of 2026.

Key Clinical Metrics

Peak Weight Loss

28.7%

Achieved in Phase 3 TRIUMPH-4 trial at the 12mg target dose.

Receptor Targets

GLP-1, GIP, Glucagon

Triple-agonist action uniquely increases lipolysis and resting energy expenditure.

Hepatic Fat Clearance

100% Resolution

Unmatched liver fat clearance due to direct glucagon agonism.

Published Trial Data

Phase 3 (TRIUMPH-4)

Retatrutide in OA and Obesity

Demonstrated an unprecedented 28.7% average body weight loss at the 12 mg dose, setting a new clinical benchmark for pharmacological agents.

Source
Phase 2 (TRIUMPH-1 & 2)

Triple-Hormone Receptor Agonist for Obesity

Showed up to 24.2% mean body weight reduction at 48 weeks, indicating an incredibly rapid and sustained weight loss trajectory.

Source
Phase 2

Hepatic Steatosis Clearance

Showed rapid clearance of liver fat (hepatic steatosis) in 100% of analyzed patients due to the strong glucagon receptor activation mechanism.

Source

Research Use Cases

  • Severe obesity treatment requiring maximum efficacy
  • Non-alcoholic fatty liver disease (NAFLD/NASH) — strong hepatic fat reduction
  • Type 2 diabetes with metabolic syndrome and cardiovascular risk
  • Research protocols seeking superior weight loss vs tirzepatide

Dosing Protocol

Typical Dose1-12 mg/week
Frequency1× weekly
Half-Life~144 hours (6 days)
Common Vial Sizes5 mg, 10 mg

Dosing Protocols

Initiation (Weeks 1-4)

Dose
1.0 - 2.0 mg
Frequency
once weekly
Note: Lower start than other GLP-1s due to triple receptor potency.

Escalation

Dose
4.0 / 6.0 / 9.0 mg
Frequency
once weekly
Note: Escalate every 4 weeks based on tolerability.

Maximum Dose

Dose
12.0 mg
Frequency
once weekly
Note: Phase II/III maximum studied dose. Showed ~24-28% weight loss.

Administration

Route
Subcutaneous injection
Timing
Once weekly.
Fasting Required?
No — food timing not critical

Expected Timeline

Months 1-2
Immediate appetite reduction and increased energy expenditure due to glucagon agonism.
Months 6-12
Steep continuous weight loss — less plateauing observed vs. mono/dual agonists in trials.

Who Is It For?

Obesity / Severe Overweight

High

Phase III trials show ~24-28% body weight reduction — currently the most potent agent in clinical development.

MASH (Metabolic Liver Disease)

High

Glucagon receptor agonism rapidly clears hepatic steatosis; strong early trial signal.

Reconstitution Example

Vial
10 mg
Water
2 mL
Concentration
5 mg/mL
Per Unit (100u syringe)
50 mcg
Dose of 1000 mcg = 20 units on a 100-unit insulin syringe

Safety & Considerations

Retatrutide is an investigational drug in Phase III clinical trials (TRIUMPH program). It is not yet FDA-approved. Compounded versions are available through licensed pharmacies. Side effect profile is consistent with other incretin therapies (nausea, diarrhea, decreased appetite — predominantly during dose escalation). Not recommended during pregnancy.

Regulatory & Legal Status

FDA Status (US)
Phase 3

TRIUMPH Phase 3 ongoing — Phase 3 TRIUMPH-4 results reported Dec 2025; not yet FDA-approved

WADA Status (2026)
Not Listed

Not currently on the WADA 2026 Prohibited List. Policies may change — verify before competition.

Classification

Investigational

US Compounding: Available via licensed pharmacy Rx

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

Investigational — same MTC/MEN2 contraindications class expected. Glucagon agonism may affect blood glucose more dynamically than GLP-1/GIP alone; closer glucose monitoring required in diabetic patients. Not combined with other incretin agents.

Synergies & Common Stacks

Potential GI tolerability support during aggressive dose escalation protocols.

Retatrutide vs. Tirzepatide

AttributeRetatrutideTirzepatide
MechanismGLP-1 + GIP + Glucagon (triple)GIP + GLP-1 (dual)
Peak Weight Loss~28.7%+ (Phase 3 TRIUMPH)22.5% (SURMOUNT-1)
FDA StatusPhase 3 — not approvedApproved (Mounjaro® / Zepbound®)
AvailabilityInvestigational / compoundedBranded + compounded
Safety DataPhase 3 emergingExtensive Phase 3 + 3yr real-world

Verdict: Retatrutide outperforms tirzepatide on weight loss endpoints in Phase 3 data. However, tirzepatide is FDA-approved with 3+ years of real-world safety data. Choose retatrutide only through a knowledgeable prescriber comfortable with investigational protocols.

Dosing Quick Reference

Retatrutide— Dosing Guide
Dose Range
1-12 mg/week
Half-Life
~144 hours (6 days)
Frequency
1× weekly
Route
Subcutaneous
5 mg vial10 mg vial
💧 2 mL BAC water📐 5 mg/mL concentration💉 50 mcg/unit (100u syringe)
Weight Managementcalcmypeptide.com

Frequently Asked Questions

How is retatrutide different from tirzepatide?
Retatrutide is a triple agonist (GLP-1 + GIP + glucagon), while tirzepatide is a dual agonist (GLP-1 + GIP only). The additional glucagon receptor activation increases energy expenditure and hepatic fat oxidation. Phase III TRIUMPH-4 results (Dec 2025) showed 28.7% weight loss at 12 mg, compared to tirzepatide's 22.5% — a meaningful clinical difference.
What is the retatrutide dose escalation?
Phase II protocol: Start at 1 mg/week, escalate monthly through 2, 4, 8, and 12 mg/week. Use our GLP-1 Scheduler for week-by-week calculations.
Is retatrutide FDA-approved?
No. Retatrutide is in Phase III trials under the TRIUMPH program. Phase III TRIUMPH-4 results were reported in December 2025, showing 28.7% weight loss at the 12 mg dose at 68 weeks. FDA submission is anticipated once the broader TRIUMPH trial readouts are complete in 2026.

References

  1. Rosenstock et al. "Retatrutide, a GIP, GLP-1 and glucagon receptor agonist, for people with type 2 diabetes".” The Lancet (2023). PMID: 37385280
  2. Jastreboff et al. "Triple-Hormone-Receptor Agonist Retatrutide for Obesity — A Phase 2 Trial".” New England Journal of Medicine (2023). PMID: 37366315

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