CalcMyPeptide
Growth HormoneAlso known as: Mod GRF(1-29), Modified GRF

CJC-1295 (no DAC)

CJC-1295 without DAC—clinically classified as Modified GRF(1-29)—is a rapidly acting Growth Hormone Releasing Hormone analog that mimics the body’s natural physiological GH rhythm. Completely avoiding the continuous "GH bleed" associated with the DAC version, Mod GRF(1-29) delivers an intense, acute spike in Growth Hormone meant to closely mirror natural nocturnal pulses. In longevity and performance protocols, it is virtually always stacked with a GHRP (like Ipamorelin) to achieve a profoundly synergistic, non-suppressive GH release.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Moderate1 peer-reviewed citation

Quick Stats

Half-Life~30 minutes
Dose Range100-300 mcg/injection
Frequency1-3× daily
Vial Sizes2 mg, 5 mg
Bioavailability~100% (subcutaneous)
Year Developed2005

Scientific Data

Molecular Formula
C130H220N42O35S
Molecular Weight
3149.55 g/mol
CAS Number
PubChem ID
Developer
ConjuChem Biotechnologies

Mechanism of Action

CJC-1295 without DAC, also known as Modified GRF(1-29) or Mod GRF, is a truncated and modified analog of GHRH (amino acids 1-29). The four amino acid substitutions protect it from enzymatic degradation, extending the biological half-life from ~7 minutes (native GHRH) to approximately 30 minutes.

Unlike the DAC version, Mod GRF creates sharp, discrete GH pulses that closely mimic natural physiology. This is why it is commonly paired with a ghrelin mimetic (like Ipamorelin) — the GHRH analog amplifies the pulse amplitude while the GHRP initiates the pulse, creating a synergistic effect that exceeds either peptide alone.

Source: PMID: 16352683

Background & History

CJC-1295 without DAC, commercially known as Modified GRF(1-29) or Mod GRF, is derived from the natural 44-amino-acid GHRH by retaining only the bioactive first 29 amino acids with four protective substitutions (Ala2→D-Ala, Gln8→Ala, Ala15→Ala, Leu27→Ala). These substitutions protect against enzymatic degradation by dipeptidyl peptidase-4 while preserving full receptor binding affinity. First described in research by Sato et al., it remains the preferred GHRH analog for physiological pulsatile GH protocols.

Research Use Cases

  • Pulsatile GH release protocols mimicking natural physiology
  • Pre-sleep GH pulse maximization for recovery and anabolism
  • Stacked with GHRPs for synergistic GH release
  • Body recomposition in GH-deficient adults

Dosing Protocol

Typical Dose100-300 mcg/injection
Frequency1-3× daily
Half-Life~30 minutes
Common Vial Sizes2 mg, 5 mg

Dosing Protocols

Standard (Solo)

Dose
100 - 300 mcg
Frequency
1-3× daily
Note: Inject on empty stomach. 1hr no food before, 30min after.

Stack with Ipamorelin

Dose
100 mcg + 100 mcg Ipamorelin
Frequency
1-3× daily
Note: Most popular GH secretagogue stack. Synergistic GHRH + GHRP effect.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 100300 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)77 mcg154 mcg231 mcg
140 lb(63 kg)90 mcg180 mcg270 mcg
160 lb(73 kg)104 mcg209 mcg313 mcg
180 lb(82 kg)117 mcg234 mcg351 mcg
200 lb(91 kg)130 mcg260 mcg390 mcg
220 lb(100 kg)143 mcg286 mcg429 mcg
250 lb(113 kg)161 mcg323 mcg484 mcg

💉 For exact syringe units based on your vial concentration, use the CJC-1295 (no DAC) Reconstitution Calculator →

Administration

Route
Subcutaneous injection
Timing
Fasted state. Best before bed, upon waking, or pre-workout. Must be on empty stomach to avoid blunting GH pulse with insulin.
Fasting Required?
Yes — inject on an empty stomach

Expected Timeline

Week 1-2
Deeper sleep, improved recovery. GH pulse initiated with each injection.
Month 1-2
Fat loss begins, lean mass preservation improves, increased sense of well-being.
Month 3+
Compounding body composition benefits. Best results when stacked with Ipamorelin.

Who Is It For?

GH Optimization (Stacked)

High

Best used in the GHRH+GHRP combo with Ipamorelin for maximal, physiological GH pulses.

Anti-Aging / Recovery

Moderate

Natural GH pulse preservation is favorable for long-term use vs. direct GH.

Reconstitution Example

Vial
2 mg
Water
2 mL
Concentration
1 mg/mL
Per Unit (100u syringe)
10 mcg
Dose of 100 mcg = 10 units on a 100-unit insulin syringe

Safety & Considerations

Research peptide. May cause facial flushing immediately after injection (common and transient). Should not be used by individuals with active cancer. Best used on an empty stomach for maximum GH pulse amplitude.

Regulatory & Legal Status

FDA Status (US)
Research Only
WADA Status (2026)
Prohibited (S2)

Competitive athletes subject to anti-doping controls should not use CJC-1295 (no DAC).

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

Must be injected on an empty stomach — insulin blunts the GH pulse. Avoid combining with CJC-1295 DAC (redundant mechanisms). Glucocorticoids suppress GH response. Evening dosing maximizes natural GH rhythm synchronization.

Synergies & Common Stacks

The gold-standard GHRH+GHRP stack. Mod GRF amplifies pulse amplitude; Ipamorelin initiates the pulse cleanly without cortisol or prolactin elevation. Typically dosed 100 mcg + 100 mcg per injection.

More potent GH release than with Ipamorelin, at the cost of some cortisol and prolactin elevation. Good for anabolic-focused protocols.

Strongest hunger drive — used when caloric intake increase is desirable alongside GH elevation.

CJC-1295 (no DAC) vs. CJC-1295 (DAC)

AttributeCJC-1295 (no DAC)CJC-1295 (DAC)
GH PatternPulsatile — natural-mimicking spikesSustained non-pulsatile GH bleed
Half-Life~30 minutes5.8–8.1 days
Dosing1–3× daily injection required1–2× weekly
Best Paired WithIpamorelin (gold-standard stack)Typically solo; optional GHRP addition
IGF-1 ProfilePhysiological — pulsatile risesSustained elevation

Verdict: CJC-1295 (No DAC) is the preferred form for users who want to replicate natural GH pulsatility and minimize sustained IGF-1 elevation. The tradeoff is more frequent injections vs. the DAC form.

Dosing Quick Reference

CJC-1295 (no DAC)— Dosing Guide
Dose Range
100-300 mcg/injection
Half-Life
~30 minutes
Frequency
1-3× daily
Route
Subcutaneous
2 mg vial5 mg vial
💧 2 mL BAC water📐 1 mg/mL concentration💉 10 mcg/unit (100u syringe)
Growth Hormonecalcmypeptide.com

Frequently Asked Questions

Why is CJC-1295 no DAC paired with Ipamorelin?
They work synergistically: CJC-1295 (GHRH analog) amplifies GH pulse amplitude, while Ipamorelin (ghrelin mimetic) initiates the pulse. Together they produce 2-5× more GH release than either peptide alone. This is the most popular GH secretagogue stack.
How should I time my CJC-1295 no DAC injections?
Inject on an empty stomach (no food for 1 hour before and 30 minutes after) to avoid insulin blunting the GH pulse. Most popular timing: before bed and/or upon waking.
What dose of CJC-1295 no DAC should I use?
Standard dose: 100-300 mcg per injection, typically 100 mcg combined with 100 mcg Ipamorelin, 1-3 times daily.

References

  1. Prakash & Goa "Growth hormone-releasing hormone and its analogs".” BioDrugs (1999). PMID: 18034534

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