CalcMyPeptide
Healing & RecoveryAlso known as: Cathelicidin, hCAP18

LL-37

LL-37 is an endogenous, highly lethal cathelicidin antimicrobial peptide serving as the human immune system's ultimate first line of defense. Vastly superior to traditional isolated antibiotics, LL-37 attacks devastating systemic infections—including severe Lyme disease, rampant Candida overgrowth, and antibiotic-resistant MRSA biofilms. Far beyond basic pathogen destruction, it acts as a hyper-vigilant immune regulator, aggressively mitigating rogue autoimmune responses, suppressing extreme systemic inflammation, and initiating advanced epithelial tissue regeneration.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Low2 peer-reviewed citations

Quick Stats

Half-Life~1-2 hours (in vivo)
Dose Range50-200 mcg/day
Frequency1× daily
Vial Sizes5 mg
BioavailabilitySubcutaneous injection
Year Developed1995

Scientific Data

Molecular Formula
C205H340N60O53S1
Molecular Weight
4493.3 g/mol
CAS Number
PubChem ID
Developer
First characterized by Agerberth et al.

Mechanism of Action

LL-37 (Cathelicidin, hCAP18) is the only human cathelicidin antimicrobial peptide — an endogenous 37-amino-acid α-helical peptide derived from the C-terminus of the hCAP18 protein. It serves as a critical first-line innate immune defense. LL-37 exerts broad-spectrum antimicrobial activity by inserting into microbial lipid bilayers, forming pores that cause rapid membrane disruption and cell lysis — effective against gram-positive and gram-negative bacteria, enveloped viruses, and fungi.

Beyond direct killing, LL-37 acts as an immunomodulator: it activates TLR4 and EGFR signaling, promotes chemotaxis of immune cells (monocytes, neutrophils, T-cells), stimulates angiogenesis, and accelerates wound healing through keratinocyte migration. It disrupts biofilms — a key advantage over antibiotics — and has been studied in chronic Lyme disease, MRSA, and as an adjuvant in cancer immunotherapy.

Source: PMID: 18082616

Background & History

LL-37 is the only known member of the cathelicidin family of antimicrobial peptides in humans, derived from the C-terminus of the hCAP18 protein. It was first described in 1995 by Gudmundsson et al. (PNAS). Beyond its broad-spectrum antimicrobial activity against bacteria, viruses, and fungi, LL-37 also modulates the immune response, promotes wound healing, and shows emerging roles in cancer biology. Its expression is induced by Vitamin D3, exercise, and infection.

Research Use Cases

  • Chronic wound healing and biofilm disruption
  • Antimicrobial support in antibiotic-resistant infections (research)
  • Immune modulation in chronic inflammatory conditions
  • Cystic fibrosis (CF) lung infection management research

Dosing Protocol

Typical Dose50-200 mcg/day
Frequency1× daily
Half-Life~4 hours (estimated)
Common Vial Sizes5 mg

Dosing Protocols

Starting Dose

Dose
50 mcg
Frequency
Daily (SC)
Note: Start low to assess tolerance. Some users experience a Herxheimer-like reaction from pathogen die-off.

Standard Protocol

Dose
100 - 150 mcg
Frequency
5 on / 2 off (SC)
Note: Most common research protocol for chronic infection and immune support.

Advanced / Infection

Dose
200 mcg
Frequency
Daily (SC)
Note: Short-term use for active infection support. Not for extended use at this dose.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 50200 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)39 mcg96 mcg154 mcg
140 lb(63 kg)45 mcg113 mcg180 mcg
160 lb(73 kg)52 mcg130 mcg209 mcg
180 lb(82 kg)59 mcg146 mcg234 mcg
200 lb(91 kg)65 mcg163 mcg260 mcg
220 lb(100 kg)71 mcg179 mcg286 mcg
250 lb(113 kg)81 mcg202 mcg323 mcg

💉 For exact syringe units based on your vial concentration, use the LL-37 Reconstitution Calculator →

Administration

Route
Subcutaneous injection
Timing
Morning. No fasting required.
Fasting Required?
No — food timing not critical

Expected Timeline

Day 3-7
Early immune activation. Possible Herxheimer-like reaction indicating pathogen response.
Week 2-4
Improved symptoms in chronic infection protocols. Enhanced wound healing at injection sites.
Month 1-3
Cumulative antimicrobial and biofilm-disruption effects in chronic infection research contexts.

Who Is It For?

Antimicrobial / Infection

Moderate

Broad-spectrum antimicrobial activity against bacteria, viruses, fungi, and biofilms. Most evidence is preclinical.

Wound Healing

Moderate

Promotes keratinocyte migration, angiogenesis, and inflammatory resolution at wound sites.

Chronic Lyme / MRSA

Low

Promising but limited human data. Use with medical supervision only.

Reconstitution Example

Vial
5 mg
Water
2.5 mL
Concentration
2 mg/mL
Per Unit (100u syringe)
20 mcg
Dose of 50 mcg = 2.5 units on a 100-unit insulin syringe

Safety & Considerations

Naturally occurring human antimicrobial peptide. Research use only — not FDA-approved as a standalone therapeutic. May cause injection site irritation. Start with 50 mcg and titrate. Herxheimer-like reactions are reported in early use.

Regulatory & Legal Status

FDA Status (US)
Research Only
WADA Status (2026)
Not Listed

Not currently on the WADA 2026 Prohibited List. Policies may change — verify before competition.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

LL-37 can cause hemolysis at high concentrations — stay within research dose ranges. Pro-inflammatory at high doses. May interact with the action of topical antibiotics (additive or competitive effects at bacterial membranes). Vitamin D deficiency reduces endogenous LL-37 production.

Synergies & Common Stacks

LL-37 provides antimicrobial protection at wound sites while BPC-157 drives vascular repair. Together supporting infected wound healing.

GHK-Cu enhances dermal matrix while LL-37 clears microbial contamination — comprehensive wound healing support.

Dosing Quick Reference

LL-37— Dosing Guide
Dose Range
50-200 mcg/day
Half-Life
~4 hours (estimated)
Frequency
1× daily
Route
Subcutaneous
5 mg vial
💧 2.5 mL BAC water📐 2 mg/mL concentration💉 20 mcg/unit (100u syringe)
Healing & Recoverycalcmypeptide.com

Frequently Asked Questions

What is LL-37 used for?
LL-37 is studied for its broad-spectrum antimicrobial properties — effective against bacteria, viruses, and fungi. It disrupts biofilms and is particularly researched for chronic infections, Lyme disease, and MRSA.
What is the LL-37 dosing protocol?
50-200 mcg daily via subcutaneous injection. Start at 50 mcg and titrate up. Many protocols use 5 days on / 2 days off to allow immune system recovery.

References

  1. Agerberth B et al. "LL-37: The Only Human Member of the Cathelicidin Family".” FEBS Letters (1995). PMID: 7556173

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