CalcMyPeptide
MitochondrialAlso known as: Mitochondrial ORF of the 12S rRNA Type-c

MOTS-c

MOTS-c is a highly advanced mitochondrial-derived peptide (MDP) literally encoded within the mitochondrial genome rather than the cell nucleus. Dubbed an "exercise mimetic," it is deployed in precision metabolic protocols to systemically reverse age-related insulin resistance and crush metabolic syndrome. By supercharging cellular bioenergetics, it dramatically enhances fatty acid oxidation, fundamentally shifting the body from glucose dependency to profound fat-burning, even in a sedentary state.

Reviewed by CalcMyPeptide Editorial Team
Last updated: April 2026Evidence: Low3 peer-reviewed citations

Quick Stats

Half-Life~16 hours
Dose Range5-10 mg/week
Frequency3-5× weekly
Vial Sizes5 mg, 10 mg
Bioavailability~100% (subcutaneous)
Year Developed2015

Scientific Data

Molecular Formula
MTNIRTLSRS... (16 aa mitochondrial peptide)
Molecular Weight
~2174 Da
CAS Number
Developer
Pinchas Cohen, University of Southern California

Mechanism of Action

MOTS-c (Mitochondrial Open Reading Frame of the 12S rRNA Type-c) is a mitochondrial-derived peptide (MDP) — a signaling molecule encoded in the mitochondrial genome rather than the nuclear genome. It activates the AMPK pathway, the master metabolic regulator that enhances cellular energy metabolism, improves insulin sensitivity, and promotes glucose uptake in skeletal muscle.

MOTS-c represents a new class of signaling molecules called "mitokines" — mitochondrial hormones that communicate metabolic status from mitochondria to the nucleus and distant tissues. It has shown exercise-mimetic effects, improving metabolic health and physical performance in animal models.

Source: PMID: 25738459

Background & History

MOTS-c is a 16-amino-acid peptide encoded within the mitochondrial 12S rRNA gene, discovered by Changhan David Lee at USC in 2015 (Cell Metabolism). It was the first regulatory peptide found to be encoded in mitochondrial DNA rather than nuclear DNA — a paradigm shift in mitochondrial biology. MOTS-c acts as a retrograde signal from mitochondria to the nucleus, activating AMPK and regulating metabolic gene expression. Exercise increases circulating MOTS-c levels, suggesting it mediates some of the metabolic benefits of physical activity.

Research Use Cases

  • Metabolic health and insulin sensitization
  • Exercise performance enhancement via AMPK activation
  • Obesity and type 2 diabetes research protocols
  • Skeletal muscle function and age-related metabolic decline
  • Longevity: mitochondrial health and lifespan extension (preclinical)

Dosing Protocol

Typical Dose5-10 mg/week
Frequency3-5× weekly
Half-Life~4-6 hours (estimated)
Common Vial Sizes5 mg, 10 mg

Dosing Protocols

Standard Protocol

Dose
5 - 10 mg
Frequency
3-5x per week (SC)
Note: 16-hour half-life supports intermittent dosing. Most rodent research used 5 mg/kg.

Body-Weight Dosing Reference

Estimated doses extrapolated from the published research range of 500010000 mcg/day (referenced to 70 kg / 154 lb). These are approximations — consult a qualified healthcare provider for personalised guidance.

WeightLowTargetHigh
120 lb(54 kg)3857 mcg5786 mcg7714 mcg
140 lb(63 kg)4500 mcg6750 mcg9000 mcg
160 lb(73 kg)5214 mcg7821 mcg10429 mcg
180 lb(82 kg)5857 mcg8786 mcg11714 mcg
200 lb(91 kg)6500 mcg9750 mcg13000 mcg
220 lb(100 kg)7143 mcg10714 mcg14286 mcg
250 lb(113 kg)8071 mcg12107 mcg16143 mcg

💉 For exact syringe units based on your vial concentration, use the MOTS-c Reconstitution Calculator →

Administration

Route
Subcutaneous injection
Timing
Morning or pre-exercise. Fasting not required.
Fasting Required?
No — food timing not critical

Expected Timeline

Week 1-4
Improved energy levels and insulin sensitivity. Exercise performance enhancement reported.
Month 1-3
Body composition improvements. Metabolic health markers may improve.

Who Is It For?

Metabolic Optimization

Moderate

AMPK activation improves glucose metabolism and insulin sensitivity. Exercise-mimetic properties.

Longevity / Anti-Aging

Low

Promising mitokine signaling in animal models. Human data is very limited.

Reconstitution Example

Vial
5 mg
Water
2 mL
Concentration
2.5 mg/mL
Per Unit (100u syringe)
25 mcg
Dose of 5000 mcg = 200 units on a 100-unit insulin syringe

Safety & Considerations

Research peptide. Limited human safety data — primarily rodent models. Naturally occurring endogenous mitochondrial peptide. Monitor blood glucose if diabetic.

Regulatory & Legal Status

FDA Status (US)
Research Only
WADA Status (2026)
Not Listed

Not currently on the WADA 2026 Prohibited List. Policies may change — verify before competition.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Interactions & Contraindications

Research compound — limited human safety data. AMPK activation may interact with metformin (additive effects on glucose metabolism). Caution with insulin or GLP-1 agonists due to additive insulin sensitization. Available in injectable form; oral bioavailability very limited.

Synergies & Common Stacks

Both are mitochondrial-derived peptides (MDPs) with overlapping cytoprotective effects. Humanin protects against apoptosis; MOTS-c enhances metabolic function. Often combined in mitochondrial health protocols.

NAD+ fuels mitochondrial function; MOTS-c activates the AMPK pathway downstream. Together they support comprehensive mitochondrial bioenergetics and metabolic resilience.

Dosing Quick Reference

MOTS-c— Dosing Guide
Dose Range
5-10 mg/week
Half-Life
~4-6 hours (estimated)
Frequency
3-5× weekly
Route
Subcutaneous
5 mg vial10 mg vial
💧 2 mL BAC water📐 2.5 mg/mL concentration💉 25 mcg/unit (100u syringe)
Mitochondrialcalcmypeptide.com

Frequently Asked Questions

What does MOTS-c do?
MOTS-c activates AMPK to improve energy metabolism, insulin sensitivity, and glucose uptake. Acts as an exercise mimetic producing metabolic benefits similar to physical training.
How is MOTS-c dosed?
5-10 mg subcutaneously, 3-5x per week. 16-hour half-life supports intermittent dosing.

References

  1. Reynolds JC et al. "MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline".” Nature Communications (2021). PMID: 33594076

📚 Related Articles

Recommended Source10% OFF

Get MOTS-c from our recommended source — independently tested, COA-verified, USP <85> endotoxin compliant. Use code 4SS6SFTUGB at checkout.

Affiliate link · Full review