Healing & Recovery

Thymosin Alpha-1

Tα1 · Zadaxin · Thymalfasin

Thymosin α1 (thymalfasin, Tα1) is a 28-amino-acid thymic peptide. Branded Zadaxin is a prescription medicine in 30+ countries, mainly for chronic hepatitis B and as an immune adjuvant. It is not FDA-approved in the United States; orphan designations are not an NDA. US research-chem and compounded vials are not that branded product. This leaf is immune biology, not a hepatitis, cancer, sepsis, or Lyme treatment page.

Not FDA-approved - research chemicalWADA (S4) - prohibited in sport at all times
Reviewed by CalcMyPeptide Editorial Team
Last updated: August 2026Evidence: Strong6 peer-reviewed citations
Typical research dose
1.6-3.2 mg twice weekly
Frequency
2× weekly
Half-life
Approximately 2 hours
Common vials
5 mg / 10 mg / 20 mg
CAS
62304-98-7
Molecular weight
3108.32 g/mol

Use-case scores

0–10 against the evidence we have, not a gym ranking. Hidden domains do not apply here.

  • Immune7.5/10

    Branded thymalfasin has human RCTs and marketing authorization in 30+ countries. Ceiling 9 for approved indication; haircut because a US research vial is not Zadaxin and this site will not score a hepatitis treatment claim.

  • Longevity / aging4/10

    Immunosenescence is the aging conversation. That is not a longevity RCT of gray-market Tα1. Community / small-human ceiling after the haircut.

Compound card

Sequence
Ac-SDAAVDTSSEITTKDLKEKKEVVEEAEN (28 aa)
Class
Thymic peptide / thymalfasin (Zadaxin is the branded drug abroad)
Formula
C129H215N33O55
Status
Not FDA-approved · research use
Dose it
5 mg + 1 mL BAC water → 5 mg/mL · 1600 mcg ≈ 32 units (U-100)
Open in calculator

Patient Suitability

✓ Best For

  • Need the 28-mer immune peptide
    This is thymalfasin class. Confirm you did not want Tβ4 or Thymalin.
  • Comparing a US vial to Zadaxin
    Use this page to keep those identities apart.

✗ Avoid If

  • Organ transplant on immunosuppressants unless the prescriber owns it
  • Active autoimmune flare unless the prescriber owns it
  • Pregnancy
  • Using it as a Lyme or cancer protocol from a forum

! Use With Caution If

  • Any autoimmune history
  • Checkpoint-inhibitor therapy (theoretically additive immune activation)
  • Unverified research-chem identity

How it works

Thymosin α1 (thymalfasin, Tα1) is a 28-residue N-acetylated peptide originally isolated from thymic extracts by Allan Goldstein. Branded Zadaxin is a prescription medicine in 30+ countries, mainly chronic hepatitis B and as an immune adjuvant. That is a real drug program. It is not an FDA-approved US product. Orphan designations (hepatitis B 1991, hepatitis C 1995, among others) are not an NDA. A research-chem or compounded US vial is not interchangeable with SciClone Zadaxin on paper.

Mechanistically it sits on innate-to-adaptive signaling: TLR2/TLR9 on dendritic cells, T-cell maturation, NK cytotoxicity, a Th1-leaning cytokine tilt in the papers that measured it. Romani 2012 is the honest “pleiotropy” review: lots of immune biology, not a single receptor drug. This catalog files it under recovery. Score it as immune. It is not BPC-157, not TB-500, and not a Lyme protocol.

Source: PMID: 17316418

Orphan designation is not FDA approval

Zadaxin is a medicine in 30+ countries. In the US, thymalfasin has orphan designations and no NDA. Do not tell a reader their research vial is FDA-approved.

Goal context

Immune competence, not a named infection

The honest job is T-cell/NK signaling. Branded labels abroad are hepatitis B and adjuvant use. A diagnosis belongs with a clinician and a labeled product, not 1.6 mg from a forum calendar.

US vial vs Zadaxin

Same 28-mer class. Different factory, label, and safety database. Compounded 503A material (when legal) is still not an NDA. Research-chem is weaker still.

Background & History

Thymosin α1 (thymalfasin) is a 28-aa thymic peptide. Zadaxin is a prescription medicine in 30+ countries, mainly chronic hepatitis B and as an immune adjuvant. It is not FDA-approved in the United States; orphan designations are not an NDA. US research-chem and compounded vials are not branded Zadaxin. Score it as immune, not as a hepatitis/cancer/Lyme treatment page.

Research Use Cases

  • ✓Defined 28-mer immune peptide identity (vs Tβ4 and Thymalin)
  • ✓Comparing a US vial to ex-US Zadaxin (they are not interchangeable on paper)
  • ✓Not a DIY viral-hepatitis, oncology, sepsis, or Lyme protocol

Dosing

PhaseDoseFrequencyNotes
Branded schedule (ex-US Zadaxin, clinician-directed)1.6 mgTwice weekly SubQCommon labeled cadence. Not a DIY hepatitis course.
US research-chem (unlabeled)1.6 mgTwice weekly SubQ (copied from the label)Copied number, not a US label. Confirm fill and sterility. Not a disease protocol.

When to increase

  • A clinician who can prescribe a real product is directing it

When to decrease

  • Injection-site or flu-like effects that are not settling
  • A clinician is directing a stop

Administration

Route / form
Subcutaneous injection (just under the skin)

Preparation

Best injection sites
Abdominal area (2 inches away from navel), Front or outer thigh, Back of upper arm
Required supplies
  • Bacteriostatic water (BAC water)
  • Insulin syringes (29-31 gauge)
  • Alcohol swabs

Storage

Before reconstitution
Keep lyophilized powder refrigerated at 36-46°F (2-8°C) for up to 2 years. Protect from light.
After reconstitution
Refrigerate at 36-46°F (2-8°C) immediately. Never freeze reconstituted solution. Use within 14-21 days.
Signs of degradation
Cloudiness or haziness • Visible particles or floaters • Color change • Unusual smell

Reconstitution

VialWaterConcentrationExample dose
5 mg1.0 mL BAC5 mg/mL1.6 mg = 0.32 mL = 32 units on a U-100 syringe
10 mg2.0 mL BAC5 mg/mL1.6 mg = 0.32 mL = 32 units on a U-100 syringe

Change vial size or water volume? Open the reconstitution calculator.

Safety & Considerations

Where Zadaxin is a labeled medicine, injection-site reactions are the common complaint and the branded safety file is larger than most research peptides. That file does not automatically transfer to an unlabeled US vial. Do not treat hepatitis, cancer, sepsis, or Lyme on this page. Transplant immunosuppression and active autoimmunity are reasons to stay out unless a physician who knows the labeled product is running it. Sterile reconstitution still matters.

Regulatory & Legal Status

FDA Status (US)
Research Only

Approved in 37+ countries (including Italy, China) as Zadaxin®; not FDA-approved in the US

WADA Status (2026)
Prohibited (S4)

Competitive athletes subject to anti-doping controls should not use Thymosin Alpha-1.

Classification

Research Chemical

US Compounding: Not eligible / not available

⚠️ This information is for educational purposes only and may not reflect the most current regulatory updates. Always verify with official FDA, WADA, and jurisdiction-specific sources before use.

Limits of current evidence

  • Not FDA-approved. Orphan designation ≠ NDA. SciClone did not complete a US hepatitis NDA that met FDA’s bar.
  • Ex-US RCTs and labels are branded thymalfasin. They do not automatically describe a research-chem cake.
  • This page will not convert adjuvant oncology, sepsis, or Lyme papers into a consumer protocol.
  • Category / compounding status in the US has moved. Check the regulatory panel; do not freeze a 2023 Category 2 sentence as eternal law without reading the current list.

Verdict

Tα1 is one of the few catalog peptides that is a real drug somewhere. Use the branded product under a clinician if the job is a labeled indication. Use this leaf to stop calling a US research vial “FDA-approved Zadaxin” and to stop treating hepatitis, cancer, sepsis, or Lyme from a reconstitution calculator.

Interactions & Contraindications

Branded thymalfasin has a larger safety file than gray-market cakes; that file does not automatically transfer. Checkpoint inhibitors: theoretically additive immune activation. Immunosuppressants: opposing job. Transplant and active autoimmunity belong with a prescriber.

Synergies & Common Stacks

Thymalin is a Khavinson thymic extract. Tα1 is a defined 28-mer. Same organ story, different material. Not a synergy RCT.

Thymosin Alpha-1 vs. TB-500

Canonical comparison: Thymosin Alpha-1 vs TB-500

AttributeThymosin Alpha-1TB-500
MechanismImmune modulation + thymic activationActin polymerization + cell migration
Primary UseImmune support, infection, cancer adjuvantPhysical tissue healing, injury recovery
Evidence QualityHuman trials (37+ country approval)Primarily preclinical
WADA StatusProhibited (S4)Prohibited (S0)
Dose Frequency900 mcg – 1.6 mg 2× weekly2–5 mg 2× weekly

Verdict: Thymosin Alpha-1 targets immunity; TB-500 targets tissue healing. These peptides address completely different goals with minimal overlap - they are not competing options but complementary tools.

What to Expect

Weeks 1-2

You Might Notice:
  • Injection-site reactions
  • Possible flu-like feel
  • Nothing dramatic is the usual report
Next Steps:
  • This is not a viral-load protocol. Keep the schedule or stop with a clinician.

Weeks 3-4

You Might Notice:
  • Tolerance at the site often improves
  • No honest consumer immune-lab schedule for a research vial
Next Steps:
  • Branded courses abroad are long. That is not a research-chem mandate.

Weeks 5-6+

You Might Notice:
  • Still not a hepatitis or oncology endpoint
Next Steps:
  • Stop or continue only with a prescriber who can use a real product

Frequently Asked Questions

Is thymosin α1 FDA-approved?▼
No. Orphan designation is not approval. Zadaxin is a medicine in 30+ countries. US research-chem and compounded vials are not that NDA.
Is a research vial the same as Zadaxin?▼
No. Same sequence class, different manufacturing, labeling, and pharmacovigilance. Do not launder branded-trial safety onto a gray-market cake.
What is the usual labeled dose abroad?▼
Zadaxin labels commonly use 1.6 mg SubQ twice weekly. That is a branded schedule, not a DIY hepatitis protocol.
How do I reconstitute a 5 mg vial?▼
This leaf’s calculator default is 5 mg + 1.0 mL BAC = 5 mg/mL. A 1.6 mg dose is 0.32 mL (32 units on a U-100). Confirm the fill on the COA before you copy that math.
Does it treat hepatitis B or C?▼
Branded thymalfasin is used for chronic hepatitis B in countries where it is approved. This site does not dose research-chem Tα1 as a viral-hepatitis drug.
Cancer, sepsis, Lyme, Long COVID?▼
Adjuvant and critical-care papers exist in the branded/clinical literature. They are not indications we will print for a research vial. See a clinician who can prescribe a real product if that is the job.
How is this different from thymosin β4 / TB-500?▼
Tα1 is immune (T-cell/NK). Tβ4 is the 43-mer actin peptide. TB-500 is usually the 7-mer fragment. Three different SKUs.
How is this different from Thymalin?▼
Thymalin is a Khavinson thymic extract. Tα1 is a defined 28-mer. Same organ story, different material.
WADA / sport?▼
Immune-modulating peptides can be prohibited depending on the list and the product. Do not assume “research” means allowed.
Storage?▼
Lyophilized refrigerated and dark. After BAC water, 2–8 °C. Discard if cloudy. Typical bacteriostatic practice is up to 28 days if sterility holds; some labels are shorter.

References

  1. Garaci E, Pica F, Matteucci C “"Historical review on thymosin α1 in oncology".” Expert Opin Biol Ther (2015). PMID: 26096345
  2. Romani L, Moretti S, Fallarino F “"Jack of all trades: thymosin α1 and its pleiotropy".” Ann N Y Acad Sci (2012). PMID: 23045964
  3. Chen JF, Chen SR, Lei ZY “"Safety and efficacy of Thymosin α1 in the treatment of hepatitis B...".” Front Immunol (2022). PMID: 35616850
  4. Ancell CD, Phipps J, Young L “"Thymosin alpha-1 (Review)".” Am J Health Syst Pharm (2001). PMID: 11381492
  5. Tuthill C, et al. “Thymosin alpha 1: a comprehensive review of the literature.” Ann N Y Acad Sci (2010). PMID: 20633111
  6. Matteucci C, et al. “Thymalfasin (thymosin-α1) in the prevention of severe infections in immunocompromised patients.” Ann N Y Acad Sci (2017). PMID: 28493411

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